Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells (2021)
Ieva Bagdonaite, Andrew J. Thompson, Xiaoning Wang, Max Søgaard, Cyrielle Fougeroux, Martin Frank, Jolene K. Diedrich, John R. Yates 3rd, Ali Salanti, Sergey Y. Vakhrushev, James C. Paulson, Hans H. Wandall
Viruses, 2021 vol.13(4):551
SNFG,
Text,
Oxford
taxonomy (1)
protein (3)
source (2)
structure (2)
composition (2)
disease (1)
reference (1)
site (38)
peptide (43)
- Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) P0DTC2
- Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) P0DTC2
- Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) P0DTC2
Protein
- O-Linked / Core 0 / GalNAc
- O-Linked / Core 1 / Gal(b1-3)GalNAc
Reported structure
- COVID-19 (DOID:0080600)
Disease
- Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
Reported glycosite
- FSNVTWFHAIHVSGTNGTK (19aa)
- FHAIHVSGTNGTKRF (15aa)
- AIHVSGTNGTKRF (13aa)
- HAIHVSGTNGTK (12aa)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- VTWFHAIHVSGTNGTK (16aa)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-63     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- HAIHVSGTNGTKRF (14aa)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- VSGTNGTKRF (10aa)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- ASTEKSNIIRGW (12aa)
- TQSLLIVNNATNVVIK (16aa)
- IVNNATNVVIKVCEF (15aa)
- YHKNNKSWMESEF (13aa)
- HKNNKSWMESEF (12aa)
- VYSSANNCTFEYVSQPFLMDLEGK (24aa)
- SALEPLVDLPIGINIT (16aa)
- VDLPIGINIT (10aa)
- TVEKGIYQTSNF (12aa)
- RVQPTESIVR (10aa)
- RVQPTESIVRFPNITNL (17aa)
- P0DTC2 Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-323     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- RVQPTESIVRFPNITNLCPF (20aa)
- GIYQTSNFRVQPTESIVR (18aa)
- GEVFNATRFASVY (13aa)
- NATRFASVY (9aa)
- VIRGDEVRQIAPGQTGKI (18aa)
- TPCNGVEGFNCY (12aa)
- P0DTC2 Thr-478     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-478     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- HAPATVCGPKKSTNL (15aa)
- NCTEVPVAIHADQLTPTWR (19aa)
- NCTEVPVAIHADQL (14aa)
- CDIPIGAGICASYQTQTNSPAR (22aa)
- QTQTNSPARAA (11aa)
- QTQTNSPARAASVASQSIIAY (21aa)
- P0DTC2 Thr-678     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→ARAA
- P0DTC2 Thr-676     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→ARAA
- QTQTNSPGSASSVASQ (16aa)
- P0DTC2 Thr-678     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→GSAS
- P0DTC2 Thr-676     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→GSAS
- QTQTNSPGSASSVASQSIIAY (21aa)
- P0DTC2 Thr-678     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→GSAS
- P0DTC2 Thr-676     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→GSAS
- FGGFNFSQILPDPSKPSKR (19aa)
- NFSQILPDPSKPS (13aa)
- NFSQILPDPSKPSKR (15aa)
- FGGFNFSQILPDPSKPSK (18aa)
- NFSQILPDPSKPSKRSF (17aa)
- DFGGFNFSQILPDPSK (16aa)
- KNFTTAPAICHDGK (14aa)
- P0DTC2 Thr-1077     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1076     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- AQEKNFTTAPAICHDGKAHFPREGVF (26aa)
- P0DTC2 Thr-1077     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1076     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1076     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1077     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- GVFVSNGTHWFVTQR (15aa)
- EGVFVSNGTHW (11aa)
- EGVFVSNGTHWFVTQR (16aa)
- P0DTC2 Ser-1097     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1100     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
Mass spectrometry observed peptide
-
- O-Linked / Core 0
(avg mass : 221.2103)
- COVID-19 (DOID:0080600)
- Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- FSNVTWFHAIHVSGTNGTK (19aa)
- FHAIHVSGTNGTKRF (15aa)
- HAIHVSGTNGTK (12aa)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- HAIHVSGTNGTKRF (14aa)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- VTWFHAIHVSGTNGTK (16aa)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-63     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- VSGTNGTKRF (10aa)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- ASTEKSNIIRGW (12aa)
- TQSLLIVNNATNVVIK (16aa)
- IVNNATNVVIKVCEF (15aa)
- YHKNNKSWMESEF (13aa)
- HKNNKSWMESEF (12aa)
- VYSSANNCTFEYVSQPFLMDLEGK (24aa)
- SALEPLVDLPIGINIT (16aa)
- VDLPIGINIT (10aa)
- TVEKGIYQTSNF (12aa)
- RVQPTESIVR (10aa)
- RVQPTESIVRFPNITNL (17aa)
- P0DTC2 Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-323     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- RVQPTESIVRFPNITNLCPF (20aa)
- GIYQTSNFRVQPTESIVR (18aa)
- GEVFNATRFASVY (13aa)
- NATRFASVY (9aa)
- HAPATVCGPKKSTNL (15aa)
- CDIPIGAGICASYQTQTNSPAR (22aa)
- QTQTNSPARAA (11aa)
- FGGFNFSQILPDPSKPSKR (19aa)
- NFSQILPDPSKPS (13aa)
- NFSQILPDPSKPSKR (15aa)
- FGGFNFSQILPDPSKPSK (18aa)
- NFSQILPDPSKPSKRSF (17aa)
- DFGGFNFSQILPDPSK (16aa)
- KNFTTAPAICHDGK (14aa)
- P0DTC2 Thr-1077     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1076     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- AQEKNFTTAPAICHDGKAHFPREGVF (26aa)
- P0DTC2 Thr-1077     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1076     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1076     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1077     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- GVFVSNGTHWFVTQR (15aa)
- EGVFVSNGTHW (11aa)
- EGVFVSNGTHWFVTQR (16aa)
- P0DTC2 Ser-1097     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1100     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
-
- O-Linked / Core 1
(avg mass : 383.3527)
- COVID-19 (DOID:0080600)
- Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- AIHVSGTNGTKRF (13aa)
- HAIHVSGTNGTK (12aa)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- VTWFHAIHVSGTNGTK (16aa)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-63     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- HAIHVSGTNGTKRF (14aa)
- P0DTC2 Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- RVQPTESIVRFPNITNL (17aa)
- P0DTC2 Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-323     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- VIRGDEVRQIAPGQTGKI (18aa)
- TPCNGVEGFNCY (12aa)
- P0DTC2 Thr-478     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-478     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- NCTEVPVAIHADQLTPTWR (19aa)
- NCTEVPVAIHADQL (14aa)
- QTQTNSPARAASVASQSIIAY (21aa)
- P0DTC2 Thr-678     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→ARAA
- P0DTC2 Thr-676     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→ARAA
- QTQTNSPGSASSVASQ (16aa)
- P0DTC2 Thr-678     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→GSAS
- P0DTC2 Thr-676     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→GSAS
- QTQTNSPGSASSVASQSIIAY (21aa)
- P0DTC2 Thr-678     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→GSAS
- P0DTC2 Thr-676     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- 682:RRAR→GSAS
- KNFTTAPAICHDGK (14aa)
- P0DTC2 Thr-1077     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1076     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- EGVFVSNGTHWFVTQR (16aa)
- P0DTC2 Ser-1097     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
- P0DTC2 Thr-1100     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
Disease
Reported glycosite
Mass spectrometry observed peptide
- O-Linked / Core 1
(avg mass : 383.3527)
Disease
Reported glycosite
Mass spectrometry observed peptide
- O-Linked / Core 0
(avg mass : 221.2103)