TQSLLIVNNATNVVIK (16aa)

Taxonomy

• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (FreeStyle 293-F) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• BTI-Tn-5B1-4 (CVCL_C190)  
• FreeStyle 293-F (CVCL_D603)  
• HEK293 (CVCL_0045)  
• HEK293-F (CVCL_6642)  

Reported structure

• N-Linked / Complex / GlcNAc(?1-?)Man(a1-3)[GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / GlcNAc(?1-?)Man(a1-3)[GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-?)]GlcNAc
• N-Linked / Complex / Structure 10031
• N-Linked / Complex / Structure 2719
• N-Linked / Complex / Gal(?1-?)GlcNAc(?1-?)Man(a1-3)[Gal(?1-?)GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / Structure 10064
• N-Linked / Complex / Structure 10021
• N-Linked / Complex / Structure 10018
• N-Linked / Complex / Gal(?1-?)GlcNAc(?1-?)Man(a1-3)[Gal(?1-?)GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc
• N-Linked / Complex / Structure 10049
• N-Linked / Complex / Structure 1147
• N-Linked / Complex / Structure 9713
• N-Linked / Complex / Structure 10015
• N-Linked / Complex / Structure 10022
• N-Linked / Complex / Structure 3507
• N-Linked / Complex / Structure 10052
• N-Linked / Complex / Structure 3446
• N-Linked / Complex / Gal(?1-?)GlcNAc(?1-?)[Gal(?1-?)GlcNAc(?1-?)]Man(a1-?)[Gal(?1-?)GlcNAc(?1-?)Man(a1-?)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / Structure 9702
• N-Linked / Complex / Structure 10009
• N-Linked / Complex / Structure 10012
• N-Linked / Complex / Structure 1523
• N-Linked / Complex / Structure 10034
• N-Linked / Complex / Structure 10020
• N-Linked / Complex / Structure 10041
• N-Linked / Complex / Gal(?1-?)GlcNAc(?1-?)[Gal(?1-?)GlcNAc(?1-?)]Man(a1-?)[Gal(?1-?)GlcNAc(?1-?)Man(a1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc
• N-Linked / Complex / Structure 9759
• N-Linked / Complex / Structure 9663
• N-Linked / Complex / Structure 10011
• N-Linked / Complex / NeuAc(?2-?)Gal(?1-?)GlcNAc(?1-?)Man(a1-3)[NeuAc(?2-?)Gal(?1-?)GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / Structure 1149
• N-Linked / Complex / Structure 10039
• N-Linked / Complex / Structure 10002
• N-Linked / Complex / Structure 10058
• N-Linked / Complex / Structure 1519
• N-Linked / Complex / Structure 720
• N-Linked / Complex / Structure 10051
• N-Linked / Complex / Structure 10036
• N-Linked / Complex / Structure 10005
• N-Linked / Complex / Structure 10061
• N-Linked / Complex / Structure 2458
• N-Linked / Complex / Structure 10035
• N-Linked / Complex / Structure 10059
• N-Linked / Complex / Structure 10063
• N-Linked / Complex / Structure 10038
• N-Linked / Complex / Structure 10044
• N-Linked / Complex / Structure 10057
• N-Linked / Complex / Structure 9997
• N-Linked / Complex / Structure 10043
• N-Linked / Complex / Structure 10040
• N-Linked / Complex / Structure 439
• N-Linked / Complex / Structure 9779
• N-Linked / Complex / Structure 138
• N-Linked / Complex / Structure 9723
• N-Linked / Complex / Structure 829
• N-Linked / Complex / Structure 10060
• N-Linked / Complex / Structure 1342
• N-Linked / Complex / Structure 10042
• N-Linked / High-Mannose / Structure 2812
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Structure 314
• N-Linked / High-Mannose / Structure 2636
• N-Linked / High-Mannose / Structure 10029
• N-Linked / Hybrid / Structure 10026
• N-Linked / Hybrid / Structure 1713
• N-Linked / Hybrid / Structure 9730
• N-Linked / Hybrid / Structure 10023
• N-Linked / Hybrid / Structure 10025
• N-Linked / Hybrid / Structure 10010
• N-Linked / Hybrid / Structure 10032
• N-Linked / Hybrid / Structure 3469
• N-Linked / Hybrid / Structure 10008
• N-Linked / Hybrid / Structure 10001
• N-Linked / Pauci-Mannose / Structure 10069
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Pauci-Mannose / Structure 9617
• N-Linked / Pauci-Mannose / Structure 10068
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ GlcNAc"
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-3)][Fuc(a1-6)]GlcNAc
• N-Linked / Pauci-Mannose / Structure 10067
• O-Linked / Core 0 / GalNAc

Composition

• HexNAc:1 (avg mass : 221.2103 )
• Hex:2 HexNAc:2 (avg mass : 748.6901 )
• Hex:2 HexNAc:2 dHex:1 (avg mass : 894.8331 )
• Hex:2 HexNAc:2 dHex:2 (avg mass : 1040.9761 )
• Hex:3 HexNAc:2 (avg mass : 910.8325 )
• Hex:3 HexNAc:2 dHex:1 (avg mass : 1056.9755 )
• Hex:3 HexNAc:2 dHex:2 (avg mass : 1203.1185 )
• Hex:3 HexNAc:3 (avg mass : 1114.0275 )
• Hex:3 HexNAc:3 dHex:1 (avg mass : 1260.1705 )
• Hex:3 HexNAc:4 (avg mass : 1317.2225 )
• Hex:3 HexNAc:4 dHex:1 (avg mass : 1463.3655 )
• Hex:3 HexNAc:5 (avg mass : 1520.4175 )
• Hex:3 HexNAc:5 dHex:1 (avg mass : 1666.5605 )
• Hex:3 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 1957.8184 )
• Hex:3 HexNAc:6 (avg mass : 1723.6125 )
• Hex:3 HexNAc:6 dHex:1 (avg mass : 1869.7555 )
• Hex:4 HexNAc:2 (avg mass : 1072.9749 )
• Hex:4 HexNAc:2 dHex:1 (avg mass : 1219.1179 )
• Hex:4 HexNAc:3 (avg mass : 1276.1699 )
• Hex:4 HexNAc:3 dHex:1 (avg mass : 1422.3129 )
• Hex:4 HexNAc:3 dHex:1 NeuAc:1 (avg mass : 1713.5708 )
• Hex:4 HexNAc:4 (avg mass : 1479.3649 )
• Hex:4 HexNAc:4 dHex:1 (avg mass : 1625.5079 )
• Hex:4 HexNAc:4 dHex:1 NeuAc:1 (avg mass : 1916.7658 )
• Hex:4 HexNAc:4 dHex:2 (avg mass : 1771.6509 )
• Hex:4 HexNAc:5 (avg mass : 1682.5599 )
• Hex:4 HexNAc:5 dHex:1 (avg mass : 1828.7029 )
• Hex:4 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 2119.9608 )
• Hex:4 HexNAc:5 dHex:1 NeuAc:2 (avg mass : 2411.2187 )
• Hex:4 HexNAc:5 dHex:2 (avg mass : 1974.8459 )
• Hex:4 HexNAc:6 (avg mass : 1885.7549 )
• Hex:4 HexNAc:6 NeuAc:1 (avg mass : 2177.0128 )
• Hex:4 HexNAc:6 dHex:1 (avg mass : 2031.8979 )
• Hex:5 HexNAc:2 (avg mass : 1235.1173 )
• Hex:5 HexNAc:3 (avg mass : 1438.3123 )
• Hex:5 HexNAc:3 NeuAc:1 (avg mass : 1729.5702 )
• Hex:5 HexNAc:3 dHex:1 (avg mass : 1584.4553 )
• Hex:5 HexNAc:4 (avg mass : 1641.5073 )
• Hex:5 HexNAc:4 NeuAc:1 (avg mass : 1932.7652 )
• Hex:5 HexNAc:4 NeuAc:2 (avg mass : 2224.0231 )
• Hex:5 HexNAc:4 dHex:1 (avg mass : 1787.6503 )
• Hex:5 HexNAc:4 dHex:1 NeuAc:1 (avg mass : 2078.9082 )
• Hex:5 HexNAc:4 dHex:1 NeuAc:2 (avg mass : 2370.1661 )
• Hex:5 HexNAc:5 (avg mass : 1844.7023 )
• Hex:5 HexNAc:5 NeuAc:1 (avg mass : 2135.9602 )
• Hex:5 HexNAc:5 NeuAc:2 (avg mass : 2427.2181 )
• Hex:5 HexNAc:5 dHex:1 (avg mass : 1990.8453 )
• Hex:5 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 2282.1032 )
• Hex:5 HexNAc:5 dHex:1 NeuAc:2 (avg mass : 2573.3611 )
• Hex:5 HexNAc:6 (avg mass : 2047.8973 )
• Hex:5 HexNAc:6 dHex:1 (avg mass : 2194.0403 )
• Hex:5 HexNAc:6 dHex:1 NeuAc:1 (avg mass : 2485.2982 )
• Hex:5 HexNAc:6 dHex:1 NeuAc:2 (avg mass : 2776.5561 )
• Hex:6 HexNAc:2 (avg mass : 1397.2597 )
• Hex:6 HexNAc:3 (avg mass : 1600.4547 )
• Hex:6 HexNAc:3 NeuAc:1 (avg mass : 1891.7126 )
• Hex:6 HexNAc:3 dHex:1 (avg mass : 1746.5977 )
• Hex:6 HexNAc:3 dHex:1 NeuAc:1 (avg mass : 2037.8556 )
• Hex:6 HexNAc:4 (avg mass : 1803.6497 )
• Hex:6 HexNAc:4 NeuAc:1 (avg mass : 2094.9076 )
• Hex:6 HexNAc:4 dHex:1 (avg mass : 1949.7927 )
• Hex:6 HexNAc:4 dHex:1 NeuAc:1 (avg mass : 2241.0506 )
• Hex:6 HexNAc:4 dHex:2 (avg mass : 2095.9357 )
• Hex:6 HexNAc:5 (avg mass : 2006.8447 )
• Hex:6 HexNAc:5 NeuAc:1 (avg mass : 2298.1026 )
• Hex:6 HexNAc:5 NeuAc:2 (avg mass : 2589.3605 )
• Hex:6 HexNAc:5 dHex:1 (avg mass : 2152.9877 )
• Hex:6 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 2444.2456 )
• Hex:6 HexNAc:5 dHex:1 NeuAc:2 (avg mass : 2735.5035 )
• Hex:6 HexNAc:5 dHex:1 NeuAc:3 (avg mass : 3026.7614 )
• Hex:6 HexNAc:6 (avg mass : 2210.0397 )
• Hex:6 HexNAc:6 NeuAc:1 (avg mass : 2501.2976 )
• Hex:6 HexNAc:6 NeuAc:2 (avg mass : 2792.5555 )
• Hex:6 HexNAc:6 dHex:1 (avg mass : 2356.1827 )
• Hex:6 HexNAc:6 dHex:1 NeuAc:1 (avg mass : 2647.4406 )
• Hex:6 HexNAc:6 dHex:1 NeuAc:2 (avg mass : 2938.6985 )
• Hex:6 HexNAc:7 (avg mass : 2413.2347 )
• Hex:7 HexNAc:2 (avg mass : 1559.4021 )
• Hex:7 HexNAc:5 dHex:1 (avg mass : 2315.1301 )
• Hex:7 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 2606.388 )
• Hex:7 HexNAc:6 (avg mass : 2372.1821 )
• Hex:7 HexNAc:6 NeuAc:1 (avg mass : 2663.44 )
• Hex:7 HexNAc:6 NeuAc:2 (avg mass : 2954.6979 )
• Hex:7 HexNAc:6 dHex:1 (avg mass : 2518.3251 )
• Hex:7 HexNAc:6 dHex:1 NeuAc:1 (avg mass : 2809.583 )
• Hex:7 HexNAc:6 dHex:1 NeuAc:2 (avg mass : 3100.8409 )
• Hex:7 HexNAc:7 dHex:1 (avg mass : 2721.5201 )
• Hex:7 HexNAc:7 dHex:1 NeuAc:2 (avg mass : 3304.0359 )
• Hex:8 HexNAc:2 (avg mass : 1721.5445 )
• Hex:9 HexNAc:2 (avg mass : 1883.6869 )

Disease

• COVID-19 (DOID:0080600)

Reference

• Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells (2021 - Ieva Bagdonaite, Andrew J. Thompson, Xiaoning Wang, Max Søgaard, Cyrielle Fougeroux, Martin Frank, Jolene K. Diedrich, John R. Yates 3rd, Ali Salanti, Sergey Y. Vakhrushev, James C. Paulson, Hans H. Wandall) / Status : Reviewed
• Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins using High-Resolution Mass Spectrometry (2020 - Yong Zhang, Wanjun Zhao, Yonghong Mao, Shisheng Wang, Yi Zhong, Tao Su, Meng Gong, Xiaofeng Lu, Jingqiu Cheng, Hao Yang) / Status : Reviewed
• Deducing the N- and O- glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2 (2020 - Asif Shajahan, Nitin T. Supekar, Anne S. Gleinich, Parastoo Azadi) / Status : Reviewed
• Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics (2020 - Dapeng Zhou, Xiaoxu Tian, Ruibing Qi, Chao Peng, Wen Zhang) / Status : Reviewed
• Site-specific glycan analysis of the SARS-CoV-2 spike (2020 - Yasunori Watanabe, Joel D. Allen, Daniel Wrapp, Jason S. McLellan, Max Crispin) / Status : Unreviewed

Reported glycosite

• Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-122
• Recombinant Spike glycoprotein (FreeStyle 293-F) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-122
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-122
• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Thr-114

Mass spectrometry observed peptide

• TQSLLIVNNATNVVIK (16aa)
  •    P0DTC2    Asn-122     Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Asn-122     Recombinant Spike glycoprotein (FreeStyle 293-F) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-114     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Asn-122     Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)