KNFTTAPAICHDGK (14aa)

Taxonomy

• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• BTI-Tn-5B1-4 (CVCL_C190)  
• HEK293 (CVCL_0045)  
• Schneider 2 (CVCL_Z232)  

Reported structure

• N-Linked / Complex / GlcNAc(?1-?)Man(a1-3)[GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Structure 10033
• N-Linked / High-Mannose / Structure 2812
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Structure 2636
• N-Linked / Pauci-Mannose / Structure 10056
• N-Linked / Pauci-Mannose / Structure 10069
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Pauci-Mannose / Structure 9617
• N-Linked / Pauci-Mannose / Structure 10068
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ GlcNAc"
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-3)][Fuc(a1-6)]GlcNAc
• N-Linked / Pauci-Mannose / Structure 10067
• N-Linked / Pauci-Mannose / Structure 10066
• O-Linked / Core 0 / GalNAc
• O-Linked / Core 1 / Gal(b1-3)GalNAc

Composition

• HexNAc:1 (avg mass : 221.2103 )
• Hex:1 HexNAc:1 (avg mass : 383.3527 )
• Hex:1 HexNAc:2 dHex:2 (avg mass : 878.8337 )
• Hex:2 HexNAc:2 dHex:1 (avg mass : 894.8331 )
• Hex:2 HexNAc:2 dHex:2 (avg mass : 1040.9761 )
• Hex:3 HexNAc:2 (avg mass : 910.8325 )
• Hex:3 HexNAc:2 dHex:1 (avg mass : 1056.9755 )
• Hex:3 HexNAc:2 dHex:2 (avg mass : 1203.1185 )
• Hex:3 HexNAc:3 (avg mass : 1114.0275 )
• Hex:3 HexNAc:3 dHex:1 (avg mass : 1260.1705 )
• Hex:3 HexNAc:3 dHex:2 (avg mass : 1406.3135 )
• Hex:3 HexNAc:4 (avg mass : 1317.2225 )
• Hex:3 HexNAc:5 dHex:1 (avg mass : 1666.5605 )
• Hex:4 HexNAc:2 (avg mass : 1072.9749 )
• Hex:5 HexNAc:2 (avg mass : 1235.1173 )
• Hex:6 HexNAc:2 (avg mass : 1397.2597 )
• Hex:8 HexNAc:2 (avg mass : 1721.5445 )

Disease

• COVID-19 (DOID:0080600)

Reference

• Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells (2021 - Ieva Bagdonaite, Andrew J. Thompson, Xiaoning Wang, Max Søgaard, Cyrielle Fougeroux, Martin Frank, Jolene K. Diedrich, John R. Yates 3rd, Ali Salanti, Sergey Y. Vakhrushev, James C. Paulson, Hans H. Wandall) / Status : Reviewed
• Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins using High-Resolution Mass Spectrometry (2020 - Yong Zhang, Wanjun Zhao, Yonghong Mao, Shisheng Wang, Yi Zhong, Tao Su, Meng Gong, Xiaofeng Lu, Jingqiu Cheng, Hao Yang) / Status : Reviewed
• Deducing the N- and O- glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2 (2020 - Asif Shajahan, Nitin T. Supekar, Anne S. Gleinich, Parastoo Azadi) / Status : Reviewed

Reported glycosite

• Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-1074
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-1074
• Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Thr-1076
  •        Thr-1077

Mass spectrometry observed peptide

• KNFTTAPAICHDGK (14aa)
  •    P0DTC2    Thr-1076     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Asn-1074     Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-1077     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Asn-1074     Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)